The below command will DELETE and CREATE a new database. Only use it when you need to remove data from the searchable database. bp_seqfeature_load.pl -a DBI::SQLite -d Search/Search_v5_new.sqlite -f -c -v --summary B73v5.fasta chromosomes/chromosomes.gff3 Gene_Models/Gene_Models.gff3 To simply add a new track to the database run the above command WITHOUT the -c flag (-c will destroy then re-create the database). NOTE: Make sure to include --summary or else they summary plots will not be generated and you'll have to rebuild the database all over again. ****IMPORTANT****** When adding new tracks to the db, copy it first and add them to the copy, otherwise the summary statistics step will cause the GBrowse instance to hang. NOTES: - Add new searchable tracks to the above command as needed. - Non-searchable tracks should go in their own SQLite database. - When required to delete a large dataset from this db, it will sometimes be faster to re-build it without the data included. The bp_seqfeature_delete.pl script is slow as hell.