Name: Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

Link to publication: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-5109-8

NCBI bioproject ID: PRJNA398446


Category: RNA-Seq, stress

Description:
Seedling of maize(Zea mays L.) B73 inbred line were cultivated using a hydroponic system under controlled conditions. For cadmium treatment, the trifoliate seedlings were transferred 
into fresh growing solutions containing 100 μM CdCl2. After 1 hr of cadmium treatment, maize seedlings of untreated and cadmium treated roots were collected for RNA-Seq analysis.

Total RNA was extracted from maize seedling roots using a plant RNA kit (Omega, USA) following the manufacturer's instructions. 
RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 
followed by kentUtils bedGraphToBigWig.