Name:Dehydration stress memory genes of Zea mays;comparison with Arabidopsis thaliana (RNA-Seq)

Link to publication: https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-14-141

NCBI bioproject ID: PRJNA210356

Category: RNA-Seq, stress

Description: 
Zea mays L. (cultivar B73), two weeks old seedlings were used in this study.
Zea Mays seedlings were grown in potting soil in growth rooms at 22 °C with a 12-h light photoperiod and light intensity of 180 μmol m− 2 s−1.

tissue: leaves

treatment:
Watered condition, corn_water_rep1
Watered condition, corn_water_rep2

dehydration stresses were performed by air-drying for 2 h 
first drought stress (S1), corn_S1_rep1
first drought stress (S1), corn_S1_rep2

A full rehydration at 22 °C, 12-h light photoperiod and light intensity of 180 μmol m− 2 s−1, followed by third stress exposure 
third drought stress (S3), corn_S3_rep1
third drought stress (S3), corn_S3_rep1

RNA extractions were done using TRIzol reagent and following the manufacturers' suggested protocol followed by purificationby Qiagen RNeasy Mini kit. RNA-seq 
reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 followed by kentUtils bedGraphToBigWig.