Name: The maize (Zea mays ssp. mays var. B73) genome encodes 33 members of the purple acid phosphatase family

Link to publication: https://www.frontiersin.org/articles/10.3389/fpls.2015.00341/full

NCBI bioproject ID: PRJNA269060


Category: RNA-Seq, stress

Description:
The transcriptional regulation for maize Pap genes  was evaluated by generating root and leaf transcriptomes under phosphate sufficient and deprivation conditions.
Zea mays ssp. mays var. B73 was grown for 21 days post-emergence in PVC tubes with inert sand substrate. The plants were fertilized after 10 days with Hoagland solution 
containing a standard 1000 μM (+P) or a modified 10 μM (–P) concentration of KH2PO4. The plants were watered every other day to maintain the field-capacity.
The distal 10 cm of the second and third fully expanded leaves and the entire seedling root system were collected for processing.

Total RNA was extracted from the distal 10 cm of the second and third fully expanded leaves and roots using PureLink RNA Mini Kit (Ambion by Life Technologies, CA, USA)
following the manufacturer's protocols. RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 
followed by kentUtils bedGraphToBigWig.