Name: Transcriptomic Analysis Revealed the Common and Divergent Responses of Maize Seedling Leaves to Cold and Heat Stresses

Link to publication: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464670/

NCBI bioproject ID: PRJNA645274


Category: RNA-Seq, stress

Description:
Seeds of maize inbred line B73 were planted in a controlled environment with conditions (25/22 °C day/light cycle) and humidity (60% average). 
Stress was imposed when the seedlings were at the three leaf stage.The stress of gradient temperature included an extreme low-temperature (4 °C for 2 h, ELT), 
medium low-temperature (10 °C for 2 h, MLT), low-temperature (16 °C for 2 h, LT), high-temperature (37 °C for 2 h, HT), medium high-temperature (42 °C for 2 h, MHT), 
and extreme high-temperature (48 °C for 1 h, EHT) were applied, and seedlings grown under a normal temperature (25 °C, NT) were set as a control. The second leaf of each seedling
in a pot were collected as one replicate. Temperature stress was initiated at different times of the day. Three replicates were prepared for each time point  of stress treatment.  

Total RNA was extracted using the TRIZOL reagent (Invitrogen, Gaithersburg,MD,USA). RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. 
Bigwig files were generated using bedtools genomecov -split -scale 1.0 followed by kentUtils bedGraphToBigWig.