Name:Mapping responsive genomic elements to heat stress in a maize diversity panel

Link to publication: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-022-02807-7

NCBI bioproject ID: PRJNA849202


Category: RNA-Seq, stress

Description:
Maize genotype B73 was grown in a growth chamber for 14 days in 30 °C/20 °C 12h/12h day/night cycle.In the chamber, the plants were shuffled around randomly
to control the microenvironmental effect. For heat treated samples, plants were subjected to  to heat for 4 h at 40 °C and plants. Control plants were
placed in a separate chamber at 30 C parallel to the heat stress samples.The third leaf of each plant was collected after the heat treatment. 


Raw reads per library were processed. RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. 
Bigwig files were generated using bedtools genomecov -split -scale 1.0 followed by kentUtils bedGraphToBigWig.