Name: Manipulating ZmEXPA4 expression ameliorates the drought-induced prolonged anthesis and silking interval in maize

Link to publication: https://academic.oup.com/plcell/article/33/6/2058/6175065

NCBI bioproject ID: PRJNA659061


Category: RNA-Seq, stress

Description:
The developmental progress of tassels (V5 to V12) and ears(V9 to V14 ) under well-watered (WW) and water stress (WS) conditions was determine identify the effect of drought on 
inflorescence development. The transcriptomes of B73 were derived from plants grown in the field under a rain-off shelter with a retractable dome and a drip-irrigation system to duplicate real field conditions.
 Relative soil water content (SWC) was maintained in a range of 35%–50% under WW treatment, at ∼20% during WS treatment.These conditions were maintained from the vegetative 5 (V5) to the V14 stage of development.
Plantings were subjected to four different watering regimes by supplying an appropriate amount of water. The watering regimes were as follows: WW (SWC = 35%–30%); WS1 (SWC = 30%–25%); WS2 (SWC = 25%–20%); and WS3 
(SWC = 25%–15%).The axillary meristems of the developing ears were sampled. The ears (20 samples) with similar sizes of (2-5, 10, 15, 20, 50 mm in length) obtained under different levels of water regime with two 
independent biological replicates.
 

Total RNA was isolated from the samples using a TaKaRa MiniBEST Plant RNA Extraction Kit following the manufacturers instructions.
 RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 
followed by kentUtils bedGraphToBigWig.