Name: A RNA-Seq Analysis of the Response of Photosynthetic System to Low Nitrogen Supply in Maize Leaf

Link to publication:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751227/#app1-ijms-18-02624

NCBI bioproject ID: PRJNA420600


Category: RNA-Seq, stress

Description:
Seeds oif maize inbred line B73 was sterilized in 10% (v/v) H2O2 for 30 min, washed and then soaked in saturated CaSO4 solution for 8 h.
The seeds were then germinaged in the dark at room temperature. The seedlings were then  transferred to plastic bags when roots were 1cm long.
Seedlings with  two visible leaves were then transferred  to porcelain pots containing nutrient solution after the endosperm was removed. The plants were transfered
to half strength of nutrient solution to full strength of nutrient solution (containing high nitrogen).The plants were grown in a growth chamber at  
28/22 °C with a 14/10 h light/dark cycle with photosynthetic photon flux density at 250–300 µmol·m−2·s−1 at canopy height at the light cycle.

Total RNA was isolated from mature tissue of expanding leaf samples. RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. 
Bigwig files were generated using bedtools genomecov -split -scale 1.0 followed by kentUtils bedGraphToBigWig.