Name: Transcriptomic complexity in young maize primary roots in response to low water potentials

Link to publication: https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-741

NCBI bioproject ID: PRJNA226757


Category: RNA-Seq, stress

Description:
Seeds of maize imbred line B73 were surface sterilized and germinated in moist germination paper roll at 16 h light, 28°C; 8 h dark, 21°C). After germination seedlings with primary root
length of 2 to 4 cm were transferred to new paper soaked with polyethylene glycol PEG8000 Mr 7,300-9,000; Roth, Karlsruhe, Germany) solutions with a water
potential of -0.2 MPa and -0.8 MPa for mild and severe water deficit, respectively, or distilled water for control experiments. 
Other set of seedlings were incubated in aerated PEG solutuins or distilled water for 6 h and 24 h (24°C, 24 h included 8 h darkness, 18°C).

Total RNA was isolated from primary roots of control and stress treated maize plants using an oligo(dT)25 mRNA isolation kit (Dynabeads, Dynal, Oslo).
RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 
followed by kentUtils bedGraphToBigWig.