Name: Nitrate and Ammonium Affect the Overall Maize Response to Nitrogen Availability by Triggering Specific and Common Transcriptional Signatures in Roots

Link to publication: https://www.mdpi.com/1422-0067/21/2/686

NCBI bioproject ID: PRJNA594965


Category: RNA-Seq, stress

Description:
Seeds of Zea mays L. B73 were germinated at 25°C for 3 days in the dark. Germinated seeds were then pretreated in N-deprived solution for 24 h. The seeds were
then transferred to -N (negative control), NO3− 1 mM or NH4+ 1 mM supplied complete nutrient solution.Lengths of 1.5 cm of root apices from the root tip cap were sampled for processing.


Total RNA was isolated from the root tip capi using TRIzol reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA USA).
RNA-seq reads were mapped by MaizeGDB to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 
followed by kentUtils bedGraphToBigWig.