Name: RNA-Seq analysis of B73 upon infection with Fusarium graminearum


Link to publication: https://www.frontiersin.org/articles/10.3389/fpls.2021.699146/full

NCBI bioproject ID: PRJNA730310

Category: RNA-Seq, stress

Description: 
Maize seedling B73 were grown under controlled conditions. 
Two weeks seedlings were inoculated with Fusarium graminearum, for RNA-Seq, seedling stems collected at ;
 0, 12, 24 hour after infection (hai)


wild type B73_12hai
wild type B73_24hai
wild type B73_0hai


Total RNA was extracted from inoculated and uninoculated B73 using TRIzol reagent (Invitrogen) then purified with RNeasy Mini Kit(Qiagen) following 
the manufacturers' suggested protocol. RNA-seq reads were mapped by MaizeGD to Zm-B73-REFERENCE-NAM-5.0. using STAR. Bigwig files were generated using bedtools genomecov -split -scale 1.0 followed by kentUtils bedGraphToBigWig.